Abstract A superior biocompatible spherical
nucleic acid (SNA) conjugate was fabricated by grafting siRNA onto the
surface of a core composed of a spherical DNA nanostructure that we have
termed a DNA nanoclew (DC). After uptake by cultured cancer cells, SNA
nanoparticles release engrafted siRNAs by cleavage of the intracellular
Dicer enzyme. Moreover, in vitro experiments reveal that such SNAs
demonstrate potent gene knockdown at both mRNA and protein levels, while
with negligible cytotoxicity. DNA.pdf
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