Abstract Prostate cancer is one of the male
killing diseases and early detection of prostate cancer is the key for
better treatment and lower cost. However, the number of prostate cancer
cells is low at the early stage, so it is very challenging to detect.
In this study, we successfully designed and developed upconversion
immune-nanohybrids (UINBs) with sustainable stability in a physiological
environment, stable optical properties and highly specific targeting
capability for early-stage prostate cancer cell detection. The
developed UINBs were characterized by transmission electron microscopy
(TEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy
(FT-IR), dynamic light scattering (DLS) and luminescence spectroscopy.
The targeting function of the biotinylated antibody nanohybrids were
confirmed by immunofluorescence assay and western blot analysis. The
UINB system is able to specifically detect prostate cancer cells with
stable and background-free luminescent signals for highly sensitive
prostate cancer cell detection. This work demonstrates a versatile
strategy to develop UCNPs based sustainably stable UINBs for sensitive
diseased cell detection. Stable Upconversion Nanohybrid Particles for Specific Prostate Cancer Cell Immunodetection.pdf
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